【摘要】 利用根茬腐解液处理模拟研究自毒作用对耐自毒能力不同的2个豇豆品种胚根蔗糖代谢和抗氧化酶活性的影响。结果表明:与不耐自毒品种相比,耐自毒品种在自毒作用下具有相对较高的发芽率、液泡转化酶(VIN)、细胞质转化酶(CIN)和蔗糖合成酶(SUS)活性,以及较高的蔗糖、己糖含量和己糖/蔗糖比值;同时,耐自毒品种胚根在自毒作用下还具有相对较高的过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性和较低的丙二醛(MDA)含量。表明胚根蔗糖代谢以及CAT和SOD可能在豇豆耐自毒作用中发挥着重要作用。更多还原

【Abstract】 The effects of autotoxicity on germination rate,sucrose metabolism and antioxidant enzyme activities in hypocotyls of two asparagus bean(Vigna unguiculata ssp.sesquipedialis L.Verdc.) cultivars,different in autotoxicity resistance,were investigated.The liquid of decomposed asparagus bean stubs was employed to simulate the autotoxicity in asparagus bean.The results showed that,compared with susceptible cultivar,resistant cultivar maintained relatively higher germination rate and activities of vacuolar invertase(VIN),cytoplasmic invertase(CIN),and sucrose synthase(SUS) under autotoxicity stress.At the same time,resistant cultivar also had relatively higher content of sucrose and hexose,and hexose/sucrose ratio than susceptible cultivar.Meanwhile,the resistant cultivar showed higher catalase(CAT) and superoxide dismutase(SOD) activities,and consequently lower malondialdehyde(MDA) content than the susceptible cultivar under autotoxicity stress.Therefore,it is suggested that sucrose metabolism and antioxidant enzymes may play important roles in autotoxicity resistance of asparagus bean.更多还原