【摘要】 以景宁木兰花粉为材料,分别用TTC染色法、离体萌发法和过氧化物酶测定法测定景宁木兰花粉活力,结果表明,TTC染色法和过氧化物酶测定法不能准确表示景宁木兰花粉的生活力;在离体萌发法测定景宁木兰花粉活力时,培养基中添加蔗糖、硼酸对景宁木兰花粉萌发影响较大,添加5%蔗糖和0.01%硼酸比较适合景宁木兰花粉萌发;景宁木兰花粉在室温下其生命力一般保持16～20 d,4℃低温贮藏可使花粉生活力时间延长至26～30 d。更多还原

【Abstract】 Pollen viability of Magnolia sinostellata was determined by TTC,H2O2 dyeing and culturing in vitro methods.The results showed that TTC and H2O2 dyeing could not determine exactly the viability of pollen,and the sucrose concentration and boric acid had great effect on pollen viability.It concluded that it had the highest germination rate with the medium of 5% sucrose and 10 mg/L boric acid.The pollen of M.sinostellata had vitality of 16-20 days at room temperature,but had 26-30 days at low temperature(4℃).更多还原