【摘要】 目的探讨日本血吸虫感染宿主不同阶段吡喹酮治疗对肝纤维化影响,为临床选择适宜的治疗时机提供依据。方法建立日本血吸虫感染家兔模型,在感染的急性期、慢性期和晚期用吡喹酮治疗,采用ELISA法检测针对可溶性虫卵抗原(soluble egg antigen,SEA)产生的IgG抗体;放免法检测血清中透明质酸(Hyaluronic acid,HA)含量;ELISA法检测血清TGF-β1水平;RT-PCR检测肝组织中的TGF-β1mRNA水平;制作肝组织病理切片,观察肝脏的病理改变。结果与慢性期和晚期比较,在感染急性期经吡喹酮治疗后,宿主血清抗SEA IgG、HA、TGF-β1水平以及肝组织TGF-β1mRNA表达水平显著降低,肝脏纤维化程度降低及肝组织结构明显改善。结论吡喹酮在血吸虫感染急性期的疗效优于慢性期和晚期,且有预防肝纤维化形成的作用。更多还原

【Abstract】 Objective To explore the therapeutic effect of praziquantel on fibrosis in different stages of Schistosoma japonicum infection in order to provide a clinical basis for the suitable timing of treatment.Methods A rabbit model of S.japonicum infection was established and praziquantel was administered in acute,chronic,and advanced stages of infection.Anti-SEA IgG antibody and TGF-β1 in serum were detected using ELISA,and hyaluronic acid(HA) content was determined using a radioimmunological assay.TGF-β1 mRNA in liver tissues was determined with RT-PCR,and histological changes in the liver were also observed.Results Compared to those rabbits treated with praziquantel in the chronic and advanced stages,rabbits treated in the acute stage had reduced levels of IgG,HA,and TGF-β1 in serum and less TGF-β1 mRNA in the liver.These rabbits also had less liver fibrosis and markedly better liver tissue.Conclusion Praziquantel has more of an effect on rabbits with S.japonicum infection in the acute stage than in the chronic or advanced stage.Treatment with praziquantel can prevent the development of liver fibrosis.更多还原