【摘要】 目的分析约氏疟原虫感染后小鼠红细胞膜蛋白及受染红细胞与内皮细胞黏附能力的变化,为进一步探索脑型疟的发生机制提供线索。方法提取约氏疟原虫感染的BALB/c小鼠红细胞膜蛋白,进行SDS-PAGE电泳,检测红细胞膜蛋白的变化。用内皮细胞株与小鼠红细胞共同培养,观察受染红细胞与内皮细胞黏附能力的改变。结果 SDS-PAGE显示约氏疟原虫感染后的小鼠红细胞膜蛋白中含有分子质量约137ku组分,53/54ku固有膜蛋白减少。与未感染对照组比较,受染红细胞对内皮细胞的黏附能力增强约3倍,差异有统计学意义(P<0.05)。结论疟原虫感染可改变宿主红细胞膜蛋白组成,增强受染红细胞与内皮细胞的黏附力。这些变化可能与脑型疟的发生有关。更多还原

【Abstract】 Objective To analyze the effects of Plasmodium yoelii(Py) on the erythrocyte membrane proteins and cytoadherence of erythrocytes to endothelial cells from infected mice,thereby providing leads for further study of cerebral malaria(CM).Methods The erythrocyte membrane proteins from BALB/c mice infected with Py 17XL were extracted and analyzed by SDS-PAGE,and the adherence of infected erythrocytes to cultured endothelial cells was determined by co-incubating both types of cells.Results A new protein with a molecular weight(MW) of about 137 ku appeared in the infected erythrocyte membranes.In contrast,an intrinsic protein of about 53/54 ku(MW) decreased.The adherence of infected erythrocytes to cultured endothelial cells increased by about three-fold.Conclusion Py infection alters the composition of erythrocyte membrane proteins and enhances the cytoadherence of infected erythrocytes to endothelial cells.These variations may contribute to CM.更多还原