【摘要】 目的:探讨BAY11-7082对前列腺癌细胞NF-κB表达及其生物学行为的影响。方法:应用CCK-8方法、蛋白印迹、NF-κB激活-核转运检测,细胞增殖抑制实验和集落形成实验方法观察BAY11-7082作用下前列腺癌细胞NF-κB蛋白的表达,细胞生长等指标的变化。结果:BAY11-7082对前列腺癌细胞转移有明显的抑制作用。BAY11-7082处理的前列腺癌细胞内NF-κB蛋白表达降低,减弱NF-κB的激活,细胞增殖和形成集落的能力下降。结论:BAY11-7082可抑制前列腺癌细胞NF-κB的激活与表达,提示NF-κB可作为前列腺癌基因治疗的重要靶点之一。更多还原

【Abstract】 Objective:This study was designed to determine the effect of BAY 11-7082 on NF-kB expression and tumor colony formation of prostate cancer cells.Methods:Cell cytotoxicity assay kit-8 was used to detect cell death and proliferation according to the protocol.Western blot and densitometry analysis were performed to compare the expression of NF-kB in the control group with that of BAY 11-7082,an NF-kB inhibitor that is used to treat prostate cancer cells.Immunofluorescent staining was also conducted to monitor NF-kB activation and translocation in the nucleus.The effects of BAY 11-7082 on colony formation were determined by counting the number of prostate cancer cell colonies.Results:The treatment of prostate cancer cell line PC-3 cells by BAY 11-7082 inhibited cell proliferation and formed few colonies.Similarly,NF-kB protein level dramatically decreased.Its translocation in the nucleus and the activation in the PC-3 cells were disrupted because of the effects of BAY 11-7082.Conclusion:BAY 11-7082 can inhibit the aberrant activation of NF-kB in prostate cancer cells and reduce tumor metastasis.Therefore,BAY 11-7082 is a potential drug for the treatment of prostate cancer patients in clinical practice in the future.更多还原