【摘要】 目的:探讨弥漫大B细胞性淋巴瘤(DLBCL)中t(14;18)易位及BCL2表达的意义。方法:用石蜡包埋组织免疫组织化学法检测142例淋巴结、胃肠道和咽淋巴环等不同器官或部位发生的DLBCL中CD10、BCL6、MUM1和BCL2的抗原表达,按免疫表型将DLBCL分成GCB-DLBCL和非GCB-DLBCL两个亚型;聚合酶链反应(PCR)扩增75例淋巴结结内DLBCL新鲜组织中由t(14;18)易位所形成的MBR/JH和lnfr/JH重组基因。结果:142例DLBCL中,75例(52.8%)表达BCL2。分型为GCB-DLBCL的51例中,18例(35.3%)表达BCL2;分型为非GCB-DLBCL的91例中,57例(62.6%)表达BCL2,两者阳性率比较有显著性差异(P=0.002)。同时,BCL2的表达阳性率按不同器官或部位发生的DLBCL作比较,结果也存在显著性差异(P=0.01)。18.7%的淋巴结结内DLBCL中有t(14;18)易位,阳性病例主要见于GCB-DLBCL之中。结论:t(14;18)易位是GCB—DLBCL亚型的遗传学特征,BCL2表达在不同亚型和不同器官或部位发生的DLBCL中有显著性差异,更多还原

【Abstract】 Objective:This study aims to explore the significance of t(14;18 ) translocation and BCL2 expression in diffuse large B-cell lymphoma(DLBCL ).Methods:Data from 142 cases with DLBCL in various organs and sites were retrieved from the file.The expression of CD 10,Bcl6,MUM1,and BCL2 antigen was detected in paraffin-embedded tissues using immunohistochemical techniques. Germinal cell-B(GCB )-DLBCL and non-GCB-DLBCL subtypes were then identified through immunophenotyping.The occurrence of t(14;18 ) translocation,manifested as a MBR/JH or mcr/JH gene rearrangement,in 75 lymph node DLBCL cases was analyzed by polymerase chain reaction using fresh samples.Results:BCL2 expression was present in 52.8%(75/142 ) of the cases.Among the 51 total cases with a GCB subtype,18 presented BCL2 expression(35.3%),whereas in 91 of the 103 cases with a non-GCB subtype,BCL2 was expressed in 57(62.6%).The positive rate for BCL2 in the two groups differed significantly(P = 0.002 ).BCL2 expression was also significantly different in patients with DLBCL compared with patients with lesions in other organs/sites(P = 0.01 ).The t(14;18 ) translocation was seen in the lymph nodes of 18.7%of the DLBCL cases,with positive cases mainly seen in those with GCB-DLBCL. Conclusion:The t(14;18 ) translocation is the genetic feature of GCB-DLBCL.BCL2 expression shows heterogeneity in DLBCL of different subtypes and in different organs sites.更多还原