【摘要】 目的利用响应面分析法对大蓟炮制工艺进行优化。方法在单因素实验基础上选取实验因素与水平,根据中心组合(Box-Benhnken)实验设计原理采用三因素三水平的响应面分析法,确定各工艺条件的影响因素,以HPLC测定大蓟黄酮的主要成分变化为响应面和等高线,优选出大蓟炒炭的最佳工艺。结果大蓟炮制的最佳工艺为炮制时间13 min、炮制温度(310±10)℃、投药量100 g。结论响应面方法是一种有效的统计技术,它是利用实验数据,通过建立数学模型来解决受多种因素影响的最优组合问题,应用于中药炮制工艺优选方面非常有意义。更多还原

【Abstract】 OBJECTIVE To optimize the processing technology of Cirsium japonicum DC by response surface methodology(RSM).METHODS Based on single factor studies,a three-variable,three-level Box-Benhnken experimental design was used to monitor the effects of independent variables like time,temperature,and weight on the dependent variable,yield of flavonoids when Cirsium japonicum DC were processed.Response surface and contour plots with flavonoids′ yield as a function were obtained with the help of HPLC.RESULTS The optimum conditions were processing time 13 min,processing temperature(310±10)℃,and raw material weight 100 g.CONCLUSION RSM is an effective technology to optimize the processing traditional Chinese medicine.更多还原