【摘要】 目的通过N+注入和紫外诱变选育拆分环氧氯丙烷的菌株。方法从污泥中筛选得到一株拆分环氧氯丙烷的菌株并诱变选育。结果该菌株生理生化鉴定为巨大芽孢杆菌,最适生长盐度为20 g.L-1。经过N+注入和紫外诱变,该菌株的目标产物含量达到99.2%,产率达到42%。结论海洋巨大芽孢杆菌具有较好的工业应用潜力。更多还原

【Abstract】 OBJECTIVE To obtain and mutate a marine Bacillus megaterium to resolute pharmaceutical intermediate,3-chloro-1,2-epoxypropane.METHODS From marine sediment,a 3-chloro-1,2-epoxypropane-resolute strain was isolated and mutated by ultraviolet rays and N+ ion.RESULTS The strain was identified as Bacillus megaterium on the basis of biophysical and biochemical test.The optimum salinity for growth was 2%,and the result of optimum salinity showed that the microbe was a typical marine microorganism.A positive mutant exhibited excellent resolution with ee value of 99% and yield of 42%.CONCLUSION The marine bacillus megaterium shows a large potential for industrial production.更多还原