Objective To investigate the effects of dental papillae cells on the Wnt signaling in rat dental pulp stem cells(DPSC).Methods DPSC and dental papillae cells were isolated and cultured in vitro,and the co-cultured systems of DPSC and dental papillae cells were established.RT-PCR and Western blot methods were used to detect the expression of Wnt1,APC,β-catenin mRNA and protein in the Wnt signaling pathway.Results After 5 d co-culture,the results of RT-PCR and Western blot showed that the expression of Wnt1,β...