【摘要】 目的实验观察转化生长因子β激活激酶(TAK1)对滑膜细胞中基质金属蛋白酶9(mmp9)和基质金属蛋白酶抑制剂1(timp1)表达的作用。方法收集行膝关节置换术的11例类风湿关节炎(RA)患者滑膜组织标本,酶消化法对滑膜成纤维细胞(FLS)体外培养。采用转染的方法将TAK1特异的小干扰RNA(siRNA)分子导入RA患者滑膜细胞,采用实时荧光定量PCR方法分析细胞内mmp9和timp1基因的相对表达。结果用特异性siRNA沉默TAK1前后,mmp9实验组降低率为74.76%。timp1实验组沉默率31.51%。结论抑制TAK1在RA滑膜细胞中的表达,虽然同时下调timp1 mRNA和mmp9 mRNA表达,但是两者之间的平衡关系被明显打破。更多还原

【Abstract】 Objective To investigate effects of TAK1 on gene expressions of mmp9 and timp1 in human fibroblast synoviocytes.Methods The samples of synovial tissues were obtained from 11 Rheumatoid Arthritis patients during knee joint replacement surgery.The synovial fibroblasts were isolated by enzyme digestion.The chemically synthesized TAK1 siRNA was transferred into in vitro cultured synoviocytes by Lipofectamine.RT-PCR was used to detect the expressions of mmp9 and timp1 mRNA.Results The expressions of mmp9 and timp1 mRNA in the cells transfected with TAK1 siRNA were decreased by 74.76% and 31.51%,respectively.Conclusion The expressions of mmp9 and timp1 were decreased simultaneously via down-regulating the TAK1 expression,but the balance of relations between the two were broken.更多还原