【摘要】 目的:建立同时测定妇可靖胶囊中木犀草素和芍药苷含量的方法。方法:色谱柱为Chromatrex C18(200 mm×4.6 mm,5μm),流动相为甲醇-0.4%磷酸(35∶65),检测波长为230 nm(检测芍药苷)和350 nm(检测木犀草素),流速为1.0 mL·min-1。结果:木犀草素、芍药苷的进样量分别在0.065～0.325μg(r=0.999 9)、0.05～0.25μg(r=0.999 8)范围内与各自峰面积积分值呈良好线性关系;平均加样回收率分别为99.78%、100.08%,RSD分别为0.31%、0.62%(n=9)。结论:本方法简便、准确,可用于妇可靖胶囊的质量控制。更多还原

【Abstract】 OBJECTIVE:To establish a method for the simultaneous determination of luteolin and paeoniflorin in Fukejing capsule.METHODS:The Chromatrex C18(200 mm×4.6 mm,5 μm) column was used with the mobile phase consisted of methanol-0.4% phosphoric acid(35:65) at the flow rate of 1.0 mL·min-1.The detection wavelength were 230 nm for paeoniflorin and 350 nm for luteolin.RESULTS:The linear range of luteolin and paeoniflorin were 0.065～0.325 μg(r=0.999 9)and 0.05～0.25 μg(r=0.999 8),respectively.The average recovery rates were 99.78%(RSD=0.31%,n=9),100.08%(RSD=0.62%,n=9),respectively.CONCLUSION:The method is simple,accurate and can be used to quality control of Fukejing capsule.更多还原