节点文献
正交试验优选杜仲多糖提取工艺
Optimization of the Extraction Process for Eucommia ulmoides Polysaccharides by Orthogonal Test
【摘要】 目的:优选杜仲多糖的提取工艺。方法:用普通水提醇沉法和微波提取法分别提取杜仲多糖,以提取温度、提取时间、料液比、提取次数为考察因素,以多糖提取率为评价指标,采用正交试验优选工艺。结果:普通水提醇沉法的最佳工艺为温度120℃、时间2 h、料液比1∶8、次数3次;微波提取法的最佳工艺为温度50℃、时间45 min、料液比1∶20、次数3次。结论:微波提取法对杜仲多糖的提取有辅助作用,较普通水提醇沉法更优。
【Abstract】 OBJECTIVE:To optimize the extraction process for Eucommia ulmoides polysaccharides(EOP).METHODS:Aqueous extraction and microwave extraction were used for the extraction of E.ulmoides polysaccharides.The extraction technology of E.ulmoides polysaccharides was optimized by orthogonal test with extraction temperature,extraction time,solid-liquid rate and extraction times as factors using extraction rate as index.RESULTS:The optimum process conditions with aqueous extraction were as follows:extraction temperature of 120 ℃,extraction time of 2 h,solid-liquid ratio of 1:8,extracting for 3 times.The optimum process conditions with microwave extraction were as follows:extraction temperature of 50 ℃,extraction time of 45 min,solid-liquid ratio of 1:20,extracting for 3 times.CONCLUSION:Microwave-assisted extraction is helpful to extract E.ulmoides polysaccharides,and it is better than aqueous extraction.
【Key words】 Eucommia ulmoides polysaccharides; Aqueous extraction; Microwave extraction; Orthogonal test;
- 【文献出处】 中国药房 ,China Pharmacy , 编辑部邮箱 ,2012年03期
- 【分类号】TQ461
- 【被引频次】13
- 【下载频次】849