【摘要】 为研究HR土鸡中存在的不同亚型禽白血病病毒(ALV)共感染的情况,本实验分别采集46只HR土公鸡的泄殖腔棉拭子和455枚鸡蛋卵白样品,采用ELISA试剂盒检测p27抗原;并采用相应的ELISA试剂盒分别检测卵黄中J亚型ALV(ALV-J)和AB亚型ALV(ALV-AB)抗体。结果表明:HR土公鸡泄殖腔棉拭子样品中p27检出阳性率为87%(40/46),卵白检出率为74.7%(340/455);而卵黄中ALV-J和ALV-AB抗体阳性率分别为0(0/30)和80%(24/30)。无菌采集初步筛选p27抗原检测为阳性的5只HR土公鸡的抗凝血接种CEF,采用抗ALV-J和ALV-A的单克隆抗体进行IFA检测,结果显示5份样品中ALV-A和ALV-J的阳性率均为100%(5/5)。同时选取HR土鸡分离株HR332进行PCR扩增鉴定,结果表明分离株HR332存在ALV-J(HR332J)和ALV-A(HR332A)。其中,HR332J与11株ALV-J国内外参考株的同源性为92.4%～97.9%;HR332A与ALV-A参考株RSA-A、MQNCSU的同源性分别为90.1%和89.7%,与国内分离株SDAU09E2的同源性为99.0%。本研究显示,地方品种HR土鸡存在不同亚型ALV共感染,同时ALV-A和ALV-J共感染同一个鸡的现象已经存在。更多还原

【Abstract】 In the present study,the co-infection of avian leukosis virus(ALV) was investigated in HR local layer chicken flock.Fourty-six cloacal swabs and 455 egg whites were collected for p27 antigen detection,while 30 egg yolk were collected for detection of ALV-AB and ALV-J antibody by ELISA.The results showed that the p27 positive rate of cloacal swabs and egg whites were 87%(40/46) and 74.7%(340/455);the antibody against ALV-AB and ALV-J in egg yolk were 0(0/30) and 80%(24/30),respectively.ALV-A and ALV-J were isolated in cell culture and identified by immunofluorescence assay from the 5 random selected p27 positive HR local layer chickens.Of which the 924 bp(ALV-J) and 1,127 bp(ALV-A) were amplified from virus isolates of HR332J and HR332A with 2 specific pairs of primers of ALV-J and ALV-A by PCR,respectively.The sequence analysis of ALV-J gp85 gene(924 bp) indicated that the homology between isolate HR332J and 11 reference strains in GenBank更多还原