【摘要】 目的了解吉林省食品中分离的沙门氏菌血清型与脉冲场凝胶电泳(PFGE)基因分型,探索沙门氏菌的分子多态性。方法将从市售鸡肉、鸡胴体、中式凉拌菜及婴儿配方奶中分离出的10株沙门氏菌进行血清学分型、PFGE分子分型,BioNumerics version软件分析比较同源性。结果 10株沙门氏菌血清型分别为4株肠炎沙门氏菌,3株里定沙门氏菌,2株阿贡纳沙门氏菌和1株雷摩沙门氏菌。PFGE聚类分型结果主要分为4型:1型4株,相似度超过95%;2型3株,又分2个亚型,亚型间的相似度为78%;3型2株,相似度为100%;4型1株。结论吉林省食品中沙门氏菌具有不同血清学及PFGE型别,相同来源和血清型的菌株PFGE型别显示高度同源性。更多还原

【Abstract】 Objective To understand the separation of Jilin food salmonella serotype and PFGE(pulsed-field gel electrophoresis) genotyping,and to explore the molecular polymorphism of Salmonella.Methods 10 strains of Salmonella were analyzed serotypes isolated from the chicken,the chicken carcass,Chinese salad,and infant formula;to analyze molecular typing by PFGE;homology comparison by BioNumerics version software was analyzed.Results The serotypes of 10 strains included 4 strains of Salmonella enteritidis,3 strains of Salmonella reading,2 strains of Salmonella agona and 1 strain of Salmonella remo.The results of PFGE cluster were mainly divided into four types,4 strains were classified as type 1,the similarity was more than 95%;3 strains were classified as type 2,2 subtypes was divided and 78% similarity between them;2 strains were classified as type 3,similar 100%;1 strain as type 4.Conclusion Different serological and PFGE type of Salmonella are showed in Jilin food,a high degree of homology with the PFGE type of same source and serotypes of strains.更多还原