【摘要】 目的通过在不同温度条件下对霍乱弧菌生存能力的观察,探索大连市水域中霍乱弧菌在流行"间歇期"如何存活和越冬。方法在自然海水和湖水中分别接种本市分离出的埃尔托型霍乱弧菌流行株小川1b型(大E940014)和埃尔托型霍乱弧菌非流行株小川32l(大E940011),在1～2℃、5～6℃及10～12℃3个温度条件下检测霍乱弧菌生存能力。结果流行株(小川1b型)在1～2℃海水存活26 d,5～6℃存活28 d,10～12℃存活46 d。可见,10～12℃生存时间明显高于1～2℃或5～6℃(P<0.05)。结论两类菌株的生存对环境温度均很敏感,温度越低死亡越快。在自然水体中,非流行株生存时间略长于流行株;而在除菌水体中两类菌存活能力基本一致。更多还原

【Abstract】 Objective To study how Vibrio cholerae survive winter in interepidemic period in the water area of Dalian through observation of its survival ability under different temperature conditions.Method El Tor(EVC) Vibrio Cholerae epidemic strain Ogawa 1b(Dalian E940014) and El Tor(EVC) Vibrio cholerae Ogawa 32l(Dalian E940011) isolated in Dalian City were inoculated in sea water and lake water,respectively.The survival ability of Vibrio cholerae was tested at temperature of 1-2 ℃,5-6 ℃ and 10-12 ℃,respectively.Result Epidemic strain Ogawa 1b was alive for 26 days in sea water at temperature of 1-2 ℃,28 days 5-6 ℃,46 days at 10-12 ℃.It was evident that the survival ability at the temperature of 10-12 ℃ was remarkably longer than that at the temperature of 1-2 ℃ or 5-6 ℃(P<0.05).Conclusion The two strains are sensitive to the temperature of environment.The lower the temperature is,the more quickly the strains die.In nature water,the survival period of non-epidemic strain is slightly shorter than that of epidemic strain;while in bacteria free water,the survival ability of the two strains is same.更多还原