【摘要】 刺五加主要分布于中国东北地区,在临床和制药行业具有广泛应用。由于受到野生资源量的限制,必须加快刺五加的品系繁育和人工栽培。本研究采用主成分分析、偏最小二乘-判别分析和正交偏最小二乘-判别分析及代谢标识物鉴别相结合的最新研究技术,对不同性别类型的中药刺五加进行区分,力求解决中药材刺五加遗传背景复杂、不同性别类型药材的鉴别困难的问题。结果发现11个次生代谢产物贡献于两个不同性别类型的刺五加,可视为区分刺五加性别类型的生物标记物。这些生物标记物主要包括黄酮、香豆素、萜类和有机酸类,它们均是与刺五加治疗作用相关的主要活性成分。在这些有重要价值的次生代谢物中,黄酮、蒽醌和有机酸在短花丝类型刺五加中高表达,而香豆素、萜类在长花丝类型中高表达。因此,通过监测这些高表达的代谢物,可选择性地栽培具有较高药用相关成分的刺五加。更多还原

【Abstract】 Acanthopanax senticosus(Rupr.et Maxim.) Harms.(AS) is a medicinal plant which mainly distributes in Northeasternarea of China,due to the widely utilization in clinical and pharmaceutical industry,the wild resource of the plant is limited,the cultivationof the plant is in needed currently.Therefore it is of importance to select a medicinal priority for cultivating from two differentsexual type of AS.In the present study,UPLC-MS technology-based metabolic profiles was used to discriminate phenotypes.By processingthe metabolomic data with principal component analysis(PCA),partial least squares-discriminant analysis(PLS-DA) and orthogonalprojection to latent structures(OPLS) analysis,eleven secondary metabolites were found to be responsible for classing thetwo varieties of Acanthopanax senticosus,they might be taken as potential biological markers of sexual type.These marker metabolitesinclude flavone,coumarin,terpenes and organic acids,which are the main active constituents related to the therapeutic effects ofAcanthopanax senticosus.Among these valuable secondary metabolites,flavone,anthraquinone and organic acids are highly expressedin short capillament Acanthopanax senticosus,while coumarin and terpenoid are highly expressed in the long capillament variety.As aresult,by monitoring these highly expressed metabolites and through selective cultivation,an Acanthopanax senticosus with higheramounts of medicinally relevant constituents could be created.更多还原