靛玉红对喉癌Hep-2细胞增殖的影响及机制

【摘要】 目的:探讨靛玉红甲肟(IRO)对人喉癌Hep-2细胞增殖的影响及机制。方法:MTT法检测不同浓度、不同作用时间IRO对Hep-2细胞增殖活性的影响。流式细胞仪检测IRO对Hep-2细胞周期的影响。Western法检测IRO对Hep-2细胞CDK4和cyclinD1蛋白表达的影响。结果:MTT结果发现IRO对Hep-2细胞具有明显的增殖抑制作用,且表现为剂量依赖性(P<0.01)。流式检测发现,以10μmol/L的IRO处理Hep-2细胞后,细胞阻滞于G0/G1期,不同浓度组间差异存在显著性(P<0.01)。Western结果发现IRO对Hep-2细胞CDK4和cyc-linD蛋白的表达有抑制作用(P<0.01)。结论:IRO具有抑制人喉癌Hep-2细胞增殖的作用,其机制与阻滞细胞于G0/G1期有关。更多还原

【Abstract】 Objective:To study the effect of Indirubin-3’-monoxime(IRO) on human laryngeal carcinoma Hep-2 cells proliferation and to explore its mechanism.Methods:Methabenzthia zuron(MTT) was used to observe the proliferation of Hep-2 cells.Flow cytometry was used to measure the cell cycle of Hep-2 cells.Western blot was used to measure the expression of CDK4 and cyclin D1 protein in the Hep-2 cells.Results:IRO inhibited growth of Hep-2 cells in a dose-dependent and time-dependent manner(P<0.01).IRO can arrests Hep-2 cell at G0/G1 phase(P<0.05).The expression of CDK4 and cyclin D1 protein of Hep-2 cells were decreased,There were significant differences between different time groups(P<0.01).Conclusions:IRO can inhibit the human laryngeal carcinoma Hep-2 cells proliferation,its mechanism is related to arrests Hep-2 cell at G0/G1 phase and reduced CDK4 and cyclin D1 expression.更多还原