【摘要】 以草鱼为试验材料,测定不同条件下草鱼中内源性转谷氨酰胺酶(TGase)的活性。结果表明,草鱼糜中的TGase在pH8～9,25～45℃时表现出较高的活性,钙离子能激发其活性,DTT在低浓度(0～1mmol/L)范围时能增加它的活性,而NH4Cl、EDTA及一些金属离子抑制TGase的活性。更多还原

【Abstract】 The characterization of transglutaminase(TGase) from grass carp was studied under different conditions.The results showed that optimal pH and optimal temperature of transglutaminase from grass carp were 8～9 and 25～45 ℃,respectively.It could be activated by calcium ions.Dithiothreitol(DTT) can increase the TGase activity.However,NH4Cl,EDTA and some metal ions inhibit the activity of TGase from grass carp.更多还原