【摘要】 利用Affymetrix水稻60k芯片研究了聚乙二醇6000(PEG)模拟干旱时,水稻根系核仁小分子RNA(snoRNA)转录本的表达活性变化。在模拟干旱胁迫下,水稻根系snoRNA转录本表达有很强的品种特异性,干旱敏感品种爱华5号中共有23个snoRNA转录本活性发生变化且均表现为上调表达,而耐旱品种湘丰早119中仅有2个snoRNA转录本表达活性发生变化,也都为上调表达;转录活性发生变化的snoRNA转录本都为C/D框型,且在染色体上存在5处snoRNA簇;爱华5号和湘丰早119在模拟干旱胁迫下表达发生变化的snoRNA转录本有1个转录本重叠,且此转录本在爱华5号中表达活性是湘丰早119中的17.54倍。更多还原

【Abstract】 The expression level of snoRNA transcripts was investigated under PEG-stimulated drought stress by Affymetrix 60 k rice genome array.The expression of rice snoRNA transcripts was cultivar-specific under drought stress.A total of 23 snoRNA transcripts from Aihua 5 and only two snoRNA transcripts from Xiangfengzao 119 were up-regulated under the osmotic stress.The snoRNA transcripts from two cultivars were box C/D type and presented in five gene clusters on the chromosomes for these snoRNA transcripts.Moreover,an overlap transcript encoding Z274 snoRNA from two cultivars was obtained,but the changed fold of the Z274 snoRNA transcript from Aihua 5 was 16.54 fold more than that of Xiangfengzao 119.更多还原