【摘要】 目的构建肠道病毒71型(EV71)山东临沂分离株3D区的系统进化树,进行遗传进化分析,探讨3D区与神经毒力的关系。方法于2009—2010年在山东省临沂市人民医院采集手足口病(HFMD)患儿的粪便标本和咽拭子标本107份,进行EV71的分离;用Bioedit和MEGA 4对EV71山东临沂分离株SDLY1、SDLY11、SDLY48、SDLY96、SDLY107的3D区进行序列分析,参比序列选自GenBank。结果 3D区的系统发生树分析结果显示,5株临沂分离株在发生树上比较集中,SDLY11、96、107的3D区同源性最高,与CoxA16原型株G-10的系统发生关系较近,与EV71原型株BrCr/70的系统发生关系较远;3D区碱基构成比和氨基酸构成比分析显示,含量最高的碱基为A(29%),含量最高的氨基酸为亮氨酸(>10%);基因序列比对和氨基酸序列比对分析结果显示,碱基突变较多,但大多为静默突变,临沂分离株与CoxA16 G-10之间的氨基酸突变中有很大一部分是赖氨酸与精氨酸之间的突变,在SDLY107的3D区发现了3个特有的氨基酸突变:N37S、K142R、G261E。结论 EV71山东临沂分离株3D区的进化可能与CoxA16 G-10有关,突变N37S、K142R、G261E可能与EV71的神经毒力有关。更多还原

【Abstract】 Objective To establish a phylogenetic tree of enterovirus 71 strains isolated in Linyi,Shandong province based on the changes of 3D region to reveal the relationship between EV71 3D and neurovirulence.Methods Enterovirus 71 strains were isolated from 107 stool and throat swab specimens collected from Linyi People’s Hospital.The nucleotide and amino acid sequences of 3D region of the strains SDLY1,SDLY11,SDLY48,SDLY96,and SDLY107 were aligned using Bioedit and MEGA 4.The phylogenetic tree,gene distance,nucleotide composition,amino acid composition,nucleotide sequence alignment,and amino acid sequence alignment were determined.Results Phylogenetic analysis of 3D region showed that the phylogeny was close within Linyi strains,especially within SDLY11,96,107,and the phylogeny was closer between Linyi strains and CoxA16 G-10 than that between Linyi strains and standard strain BrCr/70.Analysis of nucleotide composition and amino acid composition showed that composition of nucleotide A or leucine was the highest.Alignment of nucleotide and amino acid demonstrated some mutations of K and R,and 2 specific mutations for strain SDLY107,E947D and K1873R.Conclusion Evolution of 3D region of enterovirus 71 strains isolated in Linyi may be relevant to CoxA16 G-10,and two mutations of N37S and K142R may be related to the neurovirulence of EV71.更多还原