【摘要】 目的利用自制携抗体结合前列腺癌细胞,探讨超声辐照PLGA纳米粒(PLGA-NPs)体外控释脱氧核糖核酸(DNA),靶向抑癌作用。方法通过溶剂蒸干法制备携有前列腺癌雄激素受体抗体的PLGA纳米粒(NPs-PLGA-GFP-AR),包裹有绿色荧光蛋白(GFP)作为标志;加入前列腺癌PC4-2细胞培养2h后,以脉冲波(pulsed wave,PW)、连续波(continuous wave,CW)超声条件进行体外辐照对比,共聚焦荧光显微镜下观测细胞转染效果。结果 NPs摄入未增加,声辐照后绿色荧光蛋白表达急剧明显增加;无超声辐照的癌细胞中5d后始见绿色荧光,超声辐照3d即可见绿色荧光;196 h后肿瘤细胞凋亡。PW1、2、3(Duty Factor,DF为10%、20%、30%,0.05～0.15 W/cm~2))组与无超声比较转染率差异显著(P<0.05),10%、20%、30%组间转染率无显著差异(P>0.05);连续波超声辐照CW1、2、3(DF为10、20、30%,声强为0.1～0.25 W/cm~2)与无超声组对照组差异显著(P<0.05),但癌细胞脱壁、死亡率高。结论超声辐照对NPs有体外促降解及靶向控释DNA作用。更多还原

【Abstract】 Objective To target DNA by using the antibody with androgen receptors,and to inhibit prostate cancer cells to grow.To study ultrasound irradiation PLGA(Polylactic/poly glycolic acid,)nano polymers(NPs)in vitro for the release of deoxyribonucleic acid(DNA)role.Methods We got PLGA nano polymer through the solvent method. With prostate cancer androgen receptors antibodies specific nanoparticles(NPs-PLGA-GFP-AR),package green fluorescent protein(GFP)as a symbol of;use PW(Pulsed Wave),CW(Continuous Wave)ultrasound,and different conditions for ultrasound irradiation in vitro,quantitative monitoring the NPs degradation and release of DNA,and cells transfection under Laser Scanning Confocal Microscope.Results We got the nano-particles join the cell culture of human prostate cancer C4-2 cells(PC4-2)two hours,then take ultrasound irradiation,nano-particles intake did not increase, but green fluorescent protein expression sharply increased obviously.without ultrasound exposure,GFP expression appeared on day 5 after addition of the nanoshells into cultured.In contrast,applying ultrasound exposure led to early expression of GFP protein on day 3 after addition of the nanoshells into cultured cells.With PWKDuty factor, DF is 10%)of ultrasonic irradiation;After 196 hours,the tumor cell apoptosis.No ultrasonic irradiation of control group and PW1(DF is 10%,sound intensity is 0.05 W/cm~2)PW2(DF is 20%,sound intensity is 0.10 W/cm~2),PW3 (DF is 30%,sound intensity is 0.15 W/cm~2);had significant difference(P<0.05).Between DF(10%,20%,30%) groups,transfection rate had no significant difference(P>0.05).CW groups with DF(10%,20%,30%)and the group with no ultrasound comparison transfection rate had significant difference(P<0.05),but the rates of death of cell were high.Conclusions Ultrasonic irradiation of NPs,has promote in vitro degradation and targeted cells controlled release DNA effect,therefore,is a promising non-invasive treatment system.更多还原