【摘要】 目的:了解2011年乌鲁木齐地区腹泻患儿腺病毒(AdV)的感染情况,分析该地区AdV的基因亚型,进行AdV感染分子流行病学调查。方法:采集在新疆维吾尔自治区人民医院住院和门诊≤5岁腹泻患儿粪便标本315份,采用胶体金法检测轮状病毒(RV)抗原,采用聚合酶链反应(PCR)法对上述标本进行AdV检测,并对AdV阳性标本测序明确基因型。结果:315份粪便标本中共检出AdV阳性12例,总阳性率为3.81%,低于RV检出率26.35%,AdV和RV混合感染3例。AdV阳性标本中肠道腺病毒(EAdV,F组)Ad41占2.54%(8/315),其次是非肠道腺病毒(NEAdV)占1.27%(4/315),分别为Ad1、Ad3、Ad5、Ad7、Ad31型。12份阳性标本测序结果提示该地区AdV与参考株的核苷酸序列同源性为73%～99%。结论:乌鲁木齐地区AdV感染呈全年散发流行,未表现出明显的季节性,其主要的流行株为Ad41型。更多还原

【Abstract】 Objective:To study the status of adenovirus(AdV) infections in children with diarrhea and its epidemiological characteristics and genotype distribution in Urumqi in 2011. Methods:A total of 315 fecal specimens of children(≤5 years old) with diarrhea were collected from both outpatients and inpatients in our hospital from January to December 2011.Rotavirus antigens were detected by ACON Rota-Adeno.Polymerase chain reaction was used to detect AdV in fecal specimens,and genotypes were determined by sequence analysis. Results:Among all fecal specimens,12(3.81%) were detected positive for AdV which was less than the rate of rotavirus(26.35%).The main subtypes of AdV were enteric adenovirus(subgenus F) Ad41 with a positive rate of 2.54%(8/315),followed by non-enteric adenovirus(Ad1,Ad3,Ad5,Ad7,Ad31) with a positive rate of 1.27%(4/315),and Ad40 was not detected.All the strains shared 73%-99% nucleotide sequence identity with different reference strains. Conclusion:AdVs is detected all through the year and there is no distinctly seasonal distribution.Of them Ad41 is dominant.更多还原