【摘要】 目的:探索应用雷公藤多甙片建立卵巢早衰动物模型的可行性。方法:将30只SPF级Wistar大鼠随机分为对照组和模型组。模型组大鼠每日给予50mg/kg雷公藤多甙片悬浮液灌胃14天,对照组大鼠给予等量的生理盐水灌胃。观察两组大鼠体重,子宫和卵巢湿重,动情周期,血清雌二醇、卵泡刺激素(FSH)和黄体生成素(LH)水平及卵巢和子宫的组织形态学变化。结果:模型组大鼠的动情周期表现为不同形式的紊乱;模型组大鼠体重较对照组显著增高(P>0.05),血清雌二醇水平较对照组显著降低(P<0.05),血清LH水平较对照组显著增高(P<0.05),FSH较对照组增高,但差异无统计学意义(P>0.05);子宫内膜厚度和卵巢面积较对照组显著降低(P<0.05);单位卵巢面积内卵泡数和黄体数明显减少(P<0.05),闭锁卵泡数明显增多(P<0.05)。结论:应用雷公藤多甙片建立卵巢早衰的动物模型是可行的。更多还原

【Abstract】 Objective:To explore the feasibility of applying Tripterygium wilfordii polyglycoside(TWP) to establish rat model of premature ovarian failure(POF). Methods:Thirty Wistar female rats of SPF level were randomly divided into control group and model group.The rats in model group were treated with pouring TWP suspension liquid(50mg/kg daily) into stomach for 14 days;while the rats in control group were treated with pouring normal saline of the same volume into stomach.The changes of body weight,uterine wet weight,ovarian wet weight,oestrous cycle,serum levels of estradiol,follicle stimulating hormone(FSH),luteinizing hormone(LH),ovarian histomorphological changes,and uterine histomorphological changes in the two groups were observed. Results:The manifestations of oestrous cycle of rats in model group were different types of disorders;the body weight of rats in model group was higher than that in control group(P>0.05);the serum level of estradiol in model group was significantly lower than that in control group(P<0.05);the serum level of LH in model group was significantly higher than that in control group(P<0.05);the serum level of FSH in model group was higher than that in control group,but there was no significant difference(P>0.05);the thickness of endometrium and ovarian area in model group were significantly lower than those in control group(P<0.05);the numbers of follicles and corpus luteum in unit ovarian area in model group decreased significantly(P<0.05),the number of atretic follicles increased significantly(P<0.05). Conclusion:It is feasible to establish rat model of POF with TWP.更多还原