【摘要】 目的比较结肠癌患者的癌组织与癌旁正常组织中VEGF-C、HIF-1α、p16基因的表达,探讨三者之间的相关性及它们的临床意义。方法选取100例结肠癌患者作为研究对象,通过免疫组化的方法检测VEGF-C、HIF-1α、p16基因在癌组织与癌旁正常组织中的表达情况,应用统计学软件分析三者之间以及三者与结肠癌之间的相关性,同时分析它们与结肠癌临床相关症状的相关性。结果结肠癌组织中VEGF-C、HIF-1α、p16基因的表达阳性率分别为70%、65%、69%;与癌旁组织相比三者表达的阳性率均较高,差异有统计学意义(P<0.05或P<0.01);相关性分析的结果提示VEGF-C、HIF-1α、p16基因三者的表达与结肠癌的发生有关;同时VEGF-C和HIF-1α呈正相关,p16基因和HIF-1α呈负相关,p16基因和VEGF-C之间没有相关性;VEGF-C与癌组织的淋巴转移、浸润程度、临床分期有关,HIF-1α的表达量除能反映上述临床参数外,还与癌细胞的分化程度有关,p16基因则能反映患者的肿瘤分化程度、浸润程度。结论 VEGF-C、HIF-1α、p16基因的表达情况在结肠癌的发生发展均密切相关,能反映结肠癌的临床参数。更多还原

【Abstract】 Objective To compare the VEGF-C,HIF-1α and p16 gene expression of cancer and normal tissues in patients with colon cancer to explore the correlation between them and their clinical significance.Methods A total of 100 colon cancer patients were detected by immunohistochemical method to find out VEGF-C,HIF-1α,p16 gene expression,statistics analysis software was applied to analyze their correlation while the relation of colon cancer clinical symptoms.Results The expression rates of VEGF-C,HIF-1α and p16 gene were 70%,65%,69% in colon cancer tissues,higher than normal tissues.The correlation analysis results suggested that VEGF-C,HIF-1α,and p16 genes were related with colon cancer;the relationship was positive between VEGF-C and HIF-1α,negative beteewn p16 and HIF-1α.They all had relations with clinical symptoms.Conclusion The expressions of VEGF-C,HIF-1α and p16 in colon cancer are closely related,and they can reflect the clinical parameters of colon cancer.更多还原