【摘要】 目的:研究自噬相关基因在弥漫大B细胞淋巴瘤中的表达水平及其临床意义。方法:采用实时定量聚合酶链反应法,检测40例弥漫大B细胞淋巴瘤肿瘤组织和20例淋巴结反应性增生组织中自噬相关基因ATG3、ATG5和ATG7的表达水平,分析上述3个基因在弥漫大B细胞淋巴瘤组织及对照组织中的表达差异;同时,在有完整临床资料的30例患者中,结合临床信息,分析不同表达水平与淋巴瘤分期及患者预后等方面的相关性。结果:与反应性增生的淋巴结组织相比,弥漫大B细胞淋巴瘤组织中的ATG5基因表达水平显著降低(ATG3 P=0.9046;ATG5 P=0.0336;ATG7 P=0.4376)。与ATG5基因高表达患者相比,ATG5基因低表达患者中国际预后指数评分为中-高危分期者较多见(P=0.046)。结论:弥漫大B细胞淋巴瘤患者淋巴瘤肿瘤组织存在自噬减少的现象,这可能是影响疾病进展的重要因素及治疗的潜在靶点之一。更多还原

【Abstract】 Objective To study the expression of autophagy-related genes and their clinical significance in diffuse large B-cell lymphoma.Methods Autophagy-related genes ATG3,ATG5 and ATG7 expressions were assessed by real-time PCR in 40 tumor samples of diffuse large B-cell lymphoma and 20 samples of reactive hyperplasia.The relationship between gene expression level and clinical parameter was evaluated,including disease stage and patient outcome.Results Comparing with reactive hyperplasia,the expressions of autophagy related genes in diffuse large B-cell lymphoma were significantly decreased(ATG3 P=0.9046;ATG5 P=0.0336;ATG7 P=0.4376).The down-regulation of ATG5 expression was correlated with Intermediate-High Risk International Prognostic Index(P=0.046).Conclusions Defect in autophagy is present in diffuse large B-cell lymphoma and could be an important disease progression factor and a promising therapeutic target.更多还原