【摘要】 目的:研究三阴型和非三阴型乳腺癌中叉头框蛋白A1(FOXA1)、BRCA1蛋白、P53和血管内皮生长因子(VEGF)的表达情况和预后意义。方法:收集暨南大学附属第一医院乳腺癌的标本113例,根据免疫组化检测雌激素受体、孕激素受体和HER-2的表达情况分为三阴型(A组)、luminal型(B组)及HER-2过表达型(C组)乳腺癌。应用免疫组化EnVision两步法检测3组样本FOXA1、BRCA1、P53和VEGF的表达情况。结果:FOXA1总的阳性表达率为63.7%(72/113),A组阳性表达率为45.2%(19/42),B组为88.0%(44/50),C组为42.9%(9/21),FOXA1在3组中的表达存在显著差异(P<0.01);BRCA1总的阳性表达率为47.8%(54/113),A组阳性表达率为66.7%(28/42),B组为44.0%(22/50),C组为19.0%(4/21),BRCA1在3组中的表达存在显著差异(P<0.01);FOXA1阳性表达率在Ⅰ～Ⅱ期临床分期和组织学1～2级中高于阴性表达组(P<0.05),FOXA1阳性表达率与P53、VEGF表达和复发率呈负相关(P<0.05);BRCA1阳性表达率在Ⅲ期临床分期和组织学3级中高于阴性表达组(P<0.05),BRCA1阳性表达率与P53、VEGF表达和复发率呈正相关(P<0.05)。结论:FOXA1和BRCA1在乳腺癌中表达存在差异。BRCA1可作为三阴型乳腺癌预后不良的指标。更多还原

【Abstract】 AIM: To investigate the expression of forkhead box protein A1(FOXA1) BRCA1 protein,P53 and vascular endothelial growth factor(VEGF) in triple negative breast cancer(TNBC) and non-TNBC,and the relevance with the clinicopathological parameters for evaluating the prognosis.METHODS: The tumor samples were collected from 113 cases of breast cancer patients in the First Affiliated Hospital of Jinan University,and divided into TNBC group,luminal subtype group and HER-2 overexpression subtype group by the immunohistochemical results of estrogen receptor,progesterone receptor and HER-2.EnVision two-step method was used to detect the expression of FOXA1,BRCA1,P53 and VEGF in the tumor samples.RESULTS: Total FOXA1 positive expression rate was 63.7%(72/113),with 45.2%(19/42) in TNBC,88.0%(44/50) in luminal subtype and 42.9%(9/21) in HER-2 overexpression subtype.The statistically sigfnificant difference among the 3 groups was observed(P<0.01).Total BRCA-1 positive expression rate was 47.8%(54/113),with 66.7%(28/42) in TNBC,44.0%(22/50) in luminal subtype and 19.0%(4/21) in HER-2 overexpression subtype.The statisticallysignificant difference among the 3 groups was also observed(P<0.01).In the cases of clinical stages Ⅰ～Ⅱand histological grades 1～2,FOXA1 positive rate was higher than the FOXA1 negative rate(P<0.01).Negative correlations between FOXA1 positive rate and expression of P53/VEGF,and between FOXA1 positive rate and the recurrence rate were found(P<0.05).In the cases of clinical stages Ⅱ～Ⅲ and histological grades 2～3,the BRCA1 positive rate was higher than the BRCA1 negative rate(P<0.05).Positive correlations between BRCA-1 positive rate and the expression of P53/VEGF,and between BRCA1 positive rate and the recurrence rate were also observed(P<0.05).CONCLUSION: Expression of FOXA1 and BRCA1 in breast cancer is different.BRCA1 may be an adverse prognostic indicator for triple negative breast cancer.更多还原