【摘要】 目的:探讨姜黄素对胰腺癌PANC-1细胞的影响及可能机制。方法:不同浓度的姜黄素作用于PANC-1细胞后,采用MTT法检测细胞增殖,流式细胞术检测细胞凋亡,Western blotting检测K-Ras、细胞外信号调节激酶1/2(ERK1/2)、磷酸化细胞外信号调节激酶(p-ERK1/2)、Sonic hedgehog(Shh)和胶质瘤相关癌基因同系物1(GLI1)的表达水平。结果:不同浓度的姜黄素与PANC-1细胞共培养后,浓度为30 mmol/L的姜黄素组能明显抑制PANC-1细胞的增殖,与其余各组相比,差异显著(P<0.01)。PANC-1细胞经30 mmol/L姜黄素处理后,其细胞凋亡率(37.57%)明显高于对照组(4.62%)(P<0.01)。经姜黄素处理的PANC-1细胞,其K-Ras、p-ERK、Shh和GLI1表达明显低于对照组(均P<0.01)。结论:姜黄素通过抑制Ras-ERK和Shh-GLI1信号通路的活化,抑制胰腺癌PANC-1细胞增殖,并诱导细胞凋亡。更多还原

【Abstract】 AIM: To investigate the effects of curcumin on pancreatic cancer cells and the possible molecular mechanisms.METHODS: The pancreatic cancer PANC-1 cells were treated with curcumin.Growth inhibitory rate of the cells was measured by MTT assay.Apoptosis of the cells was detected by flow cytometry.The expression levels of K-Ras,extracellular signal-regulated kinase 1/2(ERK1/2),phosphorylated ERK1/2(p-ERK1/2),Sonic hedgehog(Shh) and glioma-associated oncogene homolog 1(GLI1) were determined by Western blotting.RESULTS: The growth inhibitory rate of the cells in 30 mmol/L curcumin group was significantly different from that in other groups.Apoptotic rate in 30 mmol/L curcumin group(37.57%) was significantly higher than that in control group(4.62%).The expression levels of K-Ras,p-ERK1/2,Shh and GLI1 in curcumin groups were significantly lower than those in control group.CONCLUSION: Curcumin inhibits proliferation and induces apoptosis of pancreatic cancer cells by inhibiting Ras-ERK and Shh-GLI1 signal pathways.更多还原