【摘要】 利用RT-PCR从番茄叶片中获得脱水素基因(dehydrins)的cDNA序列,命名为SlDHN2b。该基因开放阅读框1140bp,编码380个氨基酸。蛋白序列分析表明该蛋白高度亲水。Real-timePCR分析表明该基因受盐、脱水、ABA诱导表达。通过染色体步移技术获得SlDHN2b基因的启动子,PLACE数据库分析预测SlDHN2b启动子序列中含有多种逆境相关的作用元件。Northern杂交表明该基因在叶片中表达量最高,果实、花、茎中次之,根中最少。更多还原

【Abstract】 In present study,a cDNA clone,designated SlDHN2b,was isolated from tomato by RT-PCR. The ORF of SlDHN2b was 1 140 bp and contained 380 amino acid residues. Protein sequence analysis showed that the SlDHN2b was high hydrophilic. Real-time PCR analysis indicated that mRNA accumulation of SlDHN2b was induced by salt stress,abscisic acid(ABA)and dehydration. Analysis of the promoter of PdEPF1 revealed the presence of stress-responsive elements by genome walking kit. Tissue-specific expression indicated that SlDHN2b was mainly expressed in leaves,weakly expressed in bud,flower,stem and the lowest in roots. Analysis of the promoter of SlDHN2b revealed the presence of stress-responsive elements.更多还原