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东乡野生稻回交重组系的抗旱性评价体系

Drought resistance evaluation system for backcross lines of Dongxiang common wild rice(Oryza rufipogon Griff.)

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【作者】 付学琴贺浩华文飘罗向东谢建坤

【Author】 FU Xue-qin1,2,HE Hao-hua1,WEN Piao2,LUO Xiang-dong2,XIE Jian-kun2(1Jiangxi Agricultural University,Nanchang 330045,China;2College of Life Sciences,Jiangxi Normal University,Nanchang 330022,China).

【机构】 江西农业大学江西师范大学生命科学学院

【摘要】 选用协青早B//东乡野生稻/协青早B回交重组系及其亲本共79份水稻材料,研究了水稻发芽期、苗期、孕穗期、成熟期的种子萌发抗旱指数等31个指标与东乡野生稻回交重组系抗旱性的关系.结果表明:15%PEG-6000溶液处理的相对发芽势、20%PEG-6000溶液处理的种子萌发抗旱指数和相对发芽势及水分胁迫后最长根长、苗高、根鲜质量、根干质量、根系相对含水量、萎蔫率、叶片可溶性糖含量、叶片脯氨酸含量、叶片丙二醛含量、叶片相对含水量、卷叶级别、株高、单株分蘖数、单株有效穗数、穗实粒数、着粒密度、结实率、千粒重等21个指标的相对值与抗旱系数或抗旱指数的相关性显著.通过逐步回归分析筛选出9个抗旱性鉴定指标:20%PEG-6000溶液处理的相对发芽势及水分胁迫后根干质量、根系相对含水量、叶片可溶性糖含量、叶片脯氨酸含量、叶片丙二醛含量、单株有效穗、结实率和千粒重的相对值.根据这些指标和偏相关系数,建立了不同时期的抗旱性评价(D值)方程和评价体系,可对东乡野生稻回交重组系抗旱性进行较好的评价.

【Abstract】 A total of 79 rice materials containing Dongxiang wild rice(Oryza rufipogon Griff.) backcross lines(Dwr)/Xie-qingzao B(Xqz B)//Xqz B and their parents were chosen as the test objects to study the relationships between the drought resistance of these materials and the 31 drought resistance indices at germinating stage,seedling stage,booting stage,and mature stage.The results showed that the drought resistance index or the drought resistance coefficient of these materials were significantly correlated to the relative germination energy(RGE) under 15% PEG-6000 drought stress,the germination drought resistance index(GDRI) and relative germination energy(RGE) under 20% PEG-6000 drought stress,and the relative value of maximum root length(MRL),seeding height(SH),fresh root mass(FRM),dry root mass(DRM),root relative water content(RRWC),wilting rate(WR),leaf soluble sugar content(LSSC),leaf proline content(LPC),leaf MDA content(LMDAC),leaf relative water content(LRWC),level of rolling leaf(RL),plant height(PH),tiller number per plant(TNP),productive tiller number per plant(PTNP),filled spikelets per panicle(FSP),panicle density(PD),seed setting rate(SR),and 1000-grain mass(TGM) under water stress.Through stepwise regression analysis,nine drought resistance indices including the RGE under 20% PEG-6000 drought stress and the relative values of DRM,RRWC,LSSC,LPC,LMDAC,ETNP,SR,and TGM under water stress were selected.Base on these indices and their partial correlation coefficients,the drought resistance evaluation equation(D value) and evaluation system were established,which could well assess the drought resistance of the Dongxiang common wild rice backcross lines at different growth stages.

【基金】 国家自然科学基金项目(30960189,30860120);公益性行业(农业)科研专项(201103007)资助
  • 【文献出处】 应用生态学报 ,Chinese Journal of Applied Ecology , 编辑部邮箱 ,2012年05期
  • 【分类号】S511.9
  • 【被引频次】23
  • 【下载频次】326
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