【摘要】 目的研究肠道急性移植物抗宿主病(aGVHD)黏膜通透性变化与肠黏膜病理改变以及二者之间的关系。方法32只BALB/C小鼠随机分为4组:单纯照射组(对照组)和3个时段aGVHD组(aGVHD后7、11和15天组),各8只。TBI剂量8.0Gy,剂量率1.0Gy/min。高效液相色谱-蒸发光散射检测器分析(HPLC-ELSD)检测尿液标本中乳果糖(lactulose,L)和甘露醇(mannitol,M)的排出率比值(L/M),评价各组小鼠的肠道通透性;收集空肠组织标本做病理评估。结果肠道aGVHD后7、11和15天组L/M比值均明显高于对照组(P<0.05),分别为0.509±0.353,0.717±0.012,0.762±0.014,0.291±0.053。aGVHD后7、11和15天组aGVHD严重程度评分较对照组显著增加(P<0.05),分别为0.810±0.259,1.940±0.176,2.750±0.267,0.000±0.000。各组L/M与肠道aGVHD严重程度水平呈显著相关(r=0.903,P<0.01)。结论 aGVHD后随着时间延长肠黏膜通透性逐渐增加且肠道aGVHD严重程度评分逐渐增高;aGVHD后肠道通透性与肠道aGVHD严重程度呈显著相关,尿液L/M可作为肠道aGVHD严重程度的无创评估方法。更多还原

【Abstract】 Objective To investigate the change of the intestinal permeability and the morphology,and study the relationship between them after intestinal acute graft-versus-host disease(aGVHD)in mice.Methods BALB/C mice were randomly divided into 4 groups: 3 aGVHD groups(7 day,11 day and 15 day after aGVHD) and the control group,and each group had 8 mice.Total body irradiation was: dosage 8.0Gy,dose rate 1.0Gy/min.High-performance liquid chromatography evaporative light-scattering detector(HPLC-ELSD) by using two-sugar absorption test for evaluating the intestinal permeability was developed.Urine mannitol/lactulose(L/M) excretion ratios were measured.Jejunum tissues were collected,and the degree of pathology of aGVHD was analysed.Results The urine L/M ratio was significantly increased in aGVHD groups(0.509±0.353,0.717±0.012,0.762±0.014) than the control(0.291±0.053) at 7th day,11th day,15th day after aGVHD.As for the degree of pathology of aGVHD,there was significant difference(P<0.05) between the control growp(0.00±0.000) and d7,d11,d15 groups(0.810±0.259,1.940±0.176,2.750±0.267).Conclusion The intestinal permeability is increased as well as the degree of pathology of aGVHD is increased.The urine L/M ratios was correlated with the morphological change.The determination of the L/M ratio by HPLC is considered to be a reliable method to evaluate and monitor the degree of pathology of aGVHD.更多还原