【摘要】 目的明确不同剂量冬虫夏草对四氯化碳小鼠肝纤维化的作用及对TGF-β1mRNA、Smad3蛋白表达的影响。方法四氯化碳(CCl4)皮下注射复制BALB/c小鼠肝纤维化模型,虫草组在造模同时予以不同剂量虫草煎剂,直至造模结束。HE染色观察肝组织炎症,天狼猩红染色观察肝脏胶原沉积,生化法测定肝组织羟脯氨酸(Hyp)含量,免疫组化测定Smad3在肝脏内的表达,RT-PCR测定TGF-β1mRNA在肝脏内的表达。结果与正常小鼠比较,肝纤维化小鼠肝脏肝静脉与汇管区周围肝细胞明显脂肪变性,肝脏胶原沉积,可见纤维间隔形成,肝脏Hyp含量、TGF-β1mRNA、Smad3蛋白表达量均显著增加。与模型组比较,虫草组肝脏炎症与胶原沉积减轻,TGF-β1mRNA、Smad3蛋白表达量明显降低,以虫草高剂量组为明显。结论肝纤维化BALB/c小鼠的肝脏TGF-β1mRNA、Smad3蛋白表达明显上升,虫草制剂有良好的抗肝纤维化作用,高剂量虫草有着更好的抗肝纤维化作用,其作用机制与下调Smad3蛋白及TGF-β1mRNA表达有关。更多还原

【Abstract】 Objective To explore the anti-fibrosis effect of Cordyceps and stndy its effect on the expression of TGF-β1mRNA and Smad3.Methods BALB/c mice model of liver fibrosis was replicated by subcutaneous injection of CCl4.Large and small dosage of Cordyceps decoction was given to the mice in Cordyceps intervened group when modeling.Hepatic inflammation was observed by HE staining,while hepatic collagen deposition by Sirius red stain.Hepatic hy-droxyproline(Hyp) were determined by biochemical medthod.Expression of Smad3 was observed by immunohistochemical method.TGF-β1mRNA was detected by RT-PCR.Results Compared with the normal mice,obvious fatty dgeneration around hepatic veins and portal area was seen,hepatic collage deposition and fibrosis septa were found.Hepatic Hyp content,TGF-β1mRNA and Smad3 protein expression were obviously increased.Compared with the model group,hepatic inflammation and collage deposition were lessened,protein expression of TGF-β1 mRNA and Smad3 was obviously decreased in Cordyceps intervened group.Conclusion Hepatic TGF-β1mRNA,Smad3 expression was obviously increased in liver fibrosis BALB/c mice.Cordyceps had superior anti-fibrosis.Anti-fibrosis effects of large dosage Cordyceps is better than that of small dosage Cordyceps.Its mechanism might be through down regulating the TGF-β1mRNA,Smad3 protein expression.更多还原