【摘要】 目的观察菊米提取液对人胃癌细胞株AGS细胞增殖和凋亡的影响。方法 AGS细胞与0.001、0.002、0.005、0.010mg/ml的菊米提取液共同孵育48h后,采用噻唑蓝(MTT)比色法检测细胞增殖抑制率,流式细胞术分析细胞凋亡情况,分光光度法检测caspase-3/7酶及caspase-6酶活性。结果不同浓度(0.001～0.010mg/ml)的菊米提取液对AGS细胞的生长具有明显的抑制作用,呈现出一定的剂量依赖性;含菊米提取液0.002、0.005、0.010mg/ml的药物组细胞凋亡发生率分别为(11.03±2.01)%、(15.53±1.96)%及(23.77±1.65)%,不含药的对照组细胞凋亡发生率为(1.77±0.60)%;与对照组相比,除最低浓度(0.001mg/ml)药物组以外,其余药物组(0.002、0.005、0.010mg/ml)caspase-3/7酶及caspase-6酶活性有显著增强。结论菊米提取液在体外可呈浓度依赖性抑制人胃癌细胞株AGS的增殖并通过caspase-3、6、7通路促进其凋亡。更多还原

【Abstract】 Objective To explore the effect of Jumi extract on proliferation and apoptosis of human gastric carcinoma cell line AGS.Methods AGS cells and culure media containing Jumi extract(0.001,0.002,0.005,0.01mg/ml) were co-incubated for 48h.Cell proliferation rate,cell apoptosis,caspase-3/7 and caspase-6 activity were determined by methyl thiazolyl tetrazolium(MTT) colorimetric assay,flow cytometry analysis and spectrophotometric detection,respectively.Results The proliferation of AGS cells was significantly inhibited by Jumi extract(0.001-0.01mg/ml) in a dose-dependent manner.Apoptosis rates in the cells treated with Jumi extract(0.002,0.005,0.01mg/ml) were(11.03±2.01)%,(15.53±1.96)%,and(23.77±1.65)% respectively,which were higher than that in the control group(1.77±0.60)%.Compared with the control group,except lower Jumi treated group(0.001mg/ml),caspase-3/7 and caspase-6 activity were significantly increased in all Jumi groups.Conclusion The Jumi extract can inhibit the proliferation of gastric carcinoma cell line AGS in vitro in a dose-dependent manner and promote cell apoptosis through caspase-3,caspase-7 and caspase-6 pathway.更多还原