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药用甘草离体快速繁殖体系的建立
Rapid Propagation of Medicinal Glycyrrhiza In Vitro
【摘要】 筛选合适的甘草快速繁殖技术体系,为甘草的大规模生产生物活性物质、基因工程和人工制种奠定基础。以乌拉尔甘草、黄甘草和光果甘草为供试材料,以其子叶茎段和侧芽茎段作外植体,对影响甘草快速繁殖形成和发生的因素进行研究。结果显示乌拉尔甘草诱导的丛生苗数较多,是3个供试材料中最好的;甘草适宜的外植体是子叶茎段。利用甘草子叶茎段和侧芽茎段作外植体进行快速繁殖,具有生长周期短,再生频率高,适于规模化工厂生产。
【Abstract】 To screen an appropriate system for rapid propagation of Glycyrrhiza,which is a basis for large-scale production of bioactive substances,genetic engineering and artificial seed.Using Glycyrrihiza uralensis,G.korshinskyi and G.glabra for test materials,with cotyledon stem and bud stem as explants,studying the influence factors for rapid propagation of Glycyrrhiza.The results showed that Glycyrrihiza uralensis-induced clusters seedlings are the best of three test materials,and the suitable explants are cotyledon stem.With cotyledon stem and bud stem as explants for rapid propagation,it has a short growth cycle,and high frequency of regeneration,and is suitable for large-scale production.
- 【文献出处】 药物生物技术 ,Pharmaceutical Biotechnology , 编辑部邮箱 ,2012年01期
- 【分类号】S567.71
- 【被引频次】4
- 【下载频次】157