【摘要】 目的:探讨中国沙棘粗多糖中α-葡萄糖苷酶抑制剂的体外酶动力学的特性。方法:利用酶-抑制剂筛选模型,选择沙棘粗多糖,通过改变其浓度、反应时间、pH条件、温度等进行体外酶学动力学研究。结果:沙棘粗多糖浓度在0.0098～0.625 g.L-1之间时,酶的抑制率随着浓度的增大而升高,IC50为0.031 g.L-1;浓度为0.625 g.L-1时抑制率达92.52%,且与酶结合迅速,酸碱范围广,热稳定性好。结论:沙棘粗多糖是一种高活性的竞争可逆性α-葡萄糖苷酶抑制剂,抑制常数Ki=5.26g.L-1。更多还原

【Abstract】 Objective:To investigate the characteristics of in vitro enzyme kinetics of α-glucosidase inhibitors in crude polysaccharide of Chinese seabuckthorn(Hippophae rhamnoides L.ssp.sinensis Rousi).Method:Highly active crude polysaccharide of Seabuckthorn was selected by using enzyme-inhibitor screening model,and the in vitro enzyme kinetics was investigated by changing its concentration,reaction time,pH conditions,temperature and others.Results:When Seabuckthorn polysaccharide concentration is between 0.0098 g·L-1 to 0.625 g·L-1,the enzyme inhibitory rate positively correlates with the concentration,IC50 for 0.031 g·L-1;when the concentration reaches 0.625 g·L-1,and inhibitory rate is up to 92.52%;Seabuckthorn polysaccharide rapid integrates with the enzyme,and has wide pH ranged and good thermal stability.Conclusion:Crude polysaccharide of Seabuckthorn is a highly active and competitive reversible inhibitor of α-glucosidase,with inhibition constant Ki=5.26 g·L-1.更多还原