【摘要】 研究了鸟巢蕨孢子无菌播种和常规播种两种繁殖方法。结果表明,鸟巢蕨无菌播种中孢子萌发率最高可达66.7%,原叶体在固体培养基上培养不能诱导出孢子体,而需经过振荡培养后才能诱导出孢子体;常规播种法更容易诱导出孢子体,每盆播0.02 g孢子时,每克孢子可产生孢子体4 000株以上,比无菌播种操作简便,成本低。因此,孢子常规播种法更适合于鸟巢蕨规模化生产。更多还原

【Abstract】 The spore sterile culture and routine propagation of Asplenium nidus were studied respectively.The results showed that the maximum rate of spore germination was 66.7% in the spore sterile culture;the sporophyte formation was almost impossible in solid culture mediums until the prothallus were cultured for 2 weeks in 1/2MS solution.In contrast,the sporophyte formation was easy in the spore routine propagation.When the spores were applied to soil by 0.02 g per pots,one gram of spores produced over 4 000 sporophytes.The spore routine propagation was simpler and less cost than the spore sterile culture.So the spore propagation is more suitable for the scale production with spores of A.nidus.更多还原