【摘要】 目的:建立了高效液相色谱法同时测定人血浆中苯妥英钠(DPH)、卡马西平(CBZ)的药物浓度。方法:以symmetryshieldRP18柱(5.0μm,3.9×150mm)为色谱柱。流动相为甲醇∶水为50:50,流速1.0mL.min-1。Waters2487双波长紫外检测器,检测波长210nm。苯乙酮为内标物。结果:线性范围为苯妥英钠(2.2～33.0)μg.mL-1r=0.9994;卡马西平(2.0～30.0)μg.mL-1,r=0.9998;平均回收率分别为97.5%、98.2%,高、中、低浓度样品的日间和日内测定相对标准偏差RSD<2%。结论:本法适用于苯妥英钠、卡马西平的血药浓度测定。更多还原

【Abstract】 Objective: To establish an HPLC method for determining plasma level of Phenytoin、Carbamazepine.Methods: Acetophenone was selected as the internal standard.A symmetryshield RP18 column(5μm,3.9×150mm) was used.The mobile phase,methanol-phosphate solution(50:50) was used at a flow rate 1.0 mL.min-1,The detector is Waters 2487 Dual λ Absorbance Detector,detector was set at the wavelength of 210 nm.Results: The linear relation of DPH and CBZ were in the range of 2.2～33.0 and 2.0～30.0ug/ml,respectively.The relative recoveries of DPH and CBZ were 97.5%、98.2%,respectively.The RSD within-day and between-day were less than 2%.Conclusion: This method is suitable for therapeutic drug monitoring of Phenytoin、Carbamazepine.更多还原