【摘要】 采用高效液相色谱法测定河南栽培金不换中大黄酸、大黄素的含量,色谱柱为Hy-persilC18柱(250mm×4.6mm×5μm),流动相:甲醇∶0.25%磷酸(体积比80∶20),流速:1.1mL/min,检测波长为436nm.结果:大黄酸在0.154～1.54μg/mL、大黄素在0.412～2.06μg/mL范围内其峰面积积分值与质量浓度之间呈良好的线性关系(r=0.999 6和0.999 9),平均回收率分别为100.2%、99.87%(n=6),RSD分别为1.47%、2.1%.该方法简便、准确,可用于金不换中大黄酸、大黄素含量的测定.更多还原

【Abstract】 The research establishes a HPLC method for the determination of Rhein and Emodin in Rumex obtusifolius L grown in Henan.Hypersil C18(250 mm×4.6mm,5μm)and mobile phase methanol-phosphoric acid(80∶20) were used.The flow rate was 1.1 mL/min and the UV detection was 436 nm.The result showed that the linear range of Rhein concentration was from 0.154 μg/mL to 1.54 μg/mL(r=0.9996),while Emodin was between 0.412-2.06μg/mL,and its linear relation was good(r=0.9996 and 0.9999).The average recovery rate was 100.2%,and 99.87%(n=6) with the RSD of 1.47% and 2.1%.The method is simple,accurate,reproducible and applicable to the determination of the content of Rhein and Emodin in Rumex obtusifolius L..更多还原