【摘要】 目的探讨Apelin对于氧化应激相关心肌肥大的抑制作用及可能的作用机制。方法采用5-羟色胺（5-HT）刺激新生大鼠心肌细胞诱导心肌肥大细胞,而后分成对照组、Apelin处理组、抑制剂（DETC或ATZ）处理组、联合处理组;β-液体闪烁计数仪检测[³H]亮氨酸的摄人情况;免疫组化染色分析心肌细胞的横截面积;CM-H₂DCFDA检测细胞活性氧（ROS）的生成情况;试剂盒检测细胞内H₂O₂水平、过氧化氢脂质（LPO）水平、过氧化氢酶（CAT）活性以及谷胱甘肽过氧化物酶（GPx）活性;RT-PCR检测CAT mRNA以及GPx mRNA水平。结果 （1）与对照组相比,Apelin呈浓度依赖性地降低心肌肥大细胞[³H]亮氨酸的摄入以及细胞横裁面积,且以50μmol/L浓度作用最强（P<0.05）;（2）在Apelin 50μmol/L培养下,可减少细胞内ROS水平（P<0.05）,但采用DETC预处理后能够显著抑制Apelin降低ROS合成的作用（P<0.05）;（3）Apelin 50μmol/L可减少胞内H₂O₂水平,且采用ATZ预处理后可抑制Apelin降低胞内H₂O₂的作用（P<0.05）;（4）Apelin 50μmol/L处理后的CAT活性、mRNA水平升高,而LPO、GPx的活性和mRNA水平没有差异（P>0.05）,采用ATZ预处理后可显著抑制过CAT活性、mRNA水平的升高（P<0.05）。结论 Apelin可抑制氧化应激相关的心肌肥大,可能机制是激活过氧化氢酶。更多还原

【Abstract】 Objective To explore the inhibition effect of Apelin on the oxidative stress related cardiac hypertrophy and the possibly mechanism.Methods The 5-HT was applied to induce the cardiac hypertrophic cells with neonatal rat cardiac myocytes,which was divided into control group,Apelin activation group,inhibition group（DETC or ATZ）,and combination group.Theβ-liquid scintillation counter was used to detect the[³H]leucine incorporation and imunohistochemical staining was used to analyze the cell surface area.The intracellular ROS was detected by CM-H₂DCFDA.The kits were used to detect the intracellular H₂O₂ levels,lipid peroxide（LPO）levels,catalase（CAT）and glutathione peroxidase（GPx）activity.Besides, the level of CAT mRNA and GPx mRNA levels were detected by RT-PCR.Results（1）Compared with control group,the Apelin reduced the[³H]leucine incorporation and cell surface of cardiac hypertrophic cells in a dose dependent manner with the strongest effect at 50μmol/L（P<0.05）;（2）The concentration of 50μmol/L Apelin could reduce the level of intracellular ROS（P<0.05）,but the pretreatment with DETC significantly inhibited the effects of Apelin（P<0.05）;（3） The concentration of 50μmol/L Apelin could reduce the level of intracellular H₂O₂,and the pretreatment with ATZ inhibited the effects of Apelin（P<0.05）;（4）The concentration of 50μmol/L Apelin treatment could result in the improvement of the CAT activity,mRNA levels increased,while the LPO,GPx activity and mRNA levels did not have difference（P>0.05）,while the pretreatment with ATZ significantly inhibited the CAT activity,mRNA levels increase（P<0.05）.Conclusion It demonstrated that the Apelin inhibit the oxidative stress related cardiac hypertrophy,with the possibly mechanism was the activation of catalase.更多还原