【摘要】 目的研究TIMP-3基因在肝细胞肝癌(human hepatocellular carcinoma,HCC)中的表达,探讨其在HCC发生发展中的作用及意义。方法应用免疫组化(SP法)和SYBR Green实时荧光定量聚合酶链反应检测30例HCC及对应癌旁组织以及10例正常肝组织中的TIMP-3蛋白及TIMP-3mRNA的表达情况,分析TIMP-3基因的表达与HCC临床病理参数的关系。结果 TIMP-3蛋白明确定位于肝细胞胞质内。TIMP-3mRNA和TIMP-3蛋白在HCC中的表达量分别为明显低于癌旁肝组织和正常肝组织中的表达量,差异有统计学意义(P<0.01)。统计学分析显示,在HCC中TIMP-3基因的低表达与肿瘤分化程度、血清中甲胎蛋白表达水平、是否合并HBV感染等有关(P<0.05),与患者性别等因素无关。结论 TIMP-3基因可能在HCC的发生发展中起着重要作用,其表达有望成为HCC的重要分子生物学指标。更多还原

【Abstract】 Objective To investigate the expression of TIMP-3 gene in human hepatocellular carcinoma(HCC) and discussits significance during hepatocellular carcinogenesis.Methods Expression of TIMP-3 protein in HCC and the paired paracarcinomaliver tissues from 30 HCC patients was detected by immunohistochemistry(SP method),and TIMP-3 mRNA expression was analyzed by reverse transcription polymerase chain reaction.The relationship between expression of TIMP-3 geneand clinicopathologic parameters in patients with HCC was analysed.Results Expression of TIMP-3 protein was definitely locatedin the liver cancer cell plasma.The positive rates of TIMP-3 mRNA and TIMP-3 protein in HCC were respectively which were prominently lower than those in the paired paracarcinoma liver tissues(P<0.01).Statistical analysis showed that TIMP-3 in HCC gene expression and low degree of tumor differentiation,serum alpha-fetoprotein expression,such as the merger of HBV infection(P<0.05),gender and other factors unrelated to the patient.Conclusion TIMP-3 gene may occur in the development of HCC plays an important role,its expression is expected to become important molecular targets of HCC.更多还原