【摘要】 以氟碳化合物FC-77作为模型药物,制备FC-77与β-环糊精(β-CD)包合物。考察包合物与牛血清白蛋白(BSA)的相互作用。通过紫外-可见光谱、红外光谱、热重分析等方法研究包合物的物理化学性能。通过荧光光谱法研究包合物对BSA的荧光猝灭作用。结果为FC-77与β-CD之间存在强烈的相互作用,从而增加了FC-77的稳定性和水中的溶解性。荧光猝灭结果显示FC-77分子能进入BSA的疏水区域。结论是以β-CD为介质,能显著改善FC-77的理化性能,使FC-77粉末化,便于制成多种剂型,扩大给药方式。更多还原

【Abstract】 FC-77,a kind of perfluorocarbons,was used as model drug to prepare FC-77/β-CD inclusion complex and study its effect on bovine serum albumins(BSA).The physicochemical properties of FC-77/β-CD inclusion complex were characterized by UV-vis absorption spectra,FT-IR spectra and the thermogravimetric thermal analysis.The interaction of FC-77/β-CD inclusion complex and BSA was determined by fluorescence quenching technology in fluorescence methodology.FC-77 can be formed an optimal inclusion complex with cyclodextrin due to their strong interaction,which can improve the stability of FC-77 and increase their solubility in water.Furthermore,fluorescence quenching result shows that FC-77 can bind with BSA through FC-77 molecular transferring to hydrophobic region of BSA.β-cyclodextrin,used as medium,can significantly change physicochemical properties of FC-77 and prepare powdery FC-77,which result to the enlargement of the dosage and administration methods of FC-77.更多还原