【摘要】 目的探讨S100B与癫痫的关系。方法采用侧脑室注射红藻氨酸建立SD大鼠颞叶癫痫动物模型(癫痫组,40只),在痫性发作后6、12、24、72h和1周,用ELISA方法测定血清和脑脊液中S100B的含量,用免疫组化染色法观察各组大鼠海马组织中S100B蛋白的表达。其结果与正常SD大鼠(对照组,8只)比较。结果血清和脑脊液中S100B含量在癫痫发作后6h逐渐增加,24h达高峰;海马CA3和齿状回区S100B免疫阳性胶质细胞致痫后24、72h显著增高。结论 S100B蛋白在癫痫后的海马组织中大量表达,提示S100B蛋白参与了癫痫早期的病理过程;血清和脑脊液中S100B水平可作为判断癫痫所致脑损伤的早期生化指标。更多还原

【Abstract】 Objective To investigate the relationship of S100B and epilepsy. Methods Animal models of temporal lobe epilepsy were established by intracerebroventricular injection of kainic acid(KA) in 48 male SD rats(epilepsy group).The level of S100B in serum and cerebral spinal fluid(CSF) and the expression of S100B in hippocampal tissues were measured by ELISA and immunohistochemical staining at 6,12,24,72h and 1 week after seizures.The results were compared with those of 8 SD normal rats(control group). Results The level of S100B in serum and CSF increased gradually at 6h and reached the peak at 24h after seizures.The numbers of S100B-immunopositive gliocyte increased obviously in the CA3 area and dentate gyrus of the hippocampus at 24h and 72h after seizures. Conclusion S100B is over-expreessed in hippocampal tissues after seisures, indicating that S100B participates the pristine pathological course of epilepsy.The level of S100B in serum and CSF may be used as early indicators in evaluating brain injury in epileptic rats.更多还原