【摘要】 目的探讨细胞分裂周期蛋白25同源蛋白C(CDC25C)基因在肾透明细胞癌(ccRCC)组织中的表达水平及其临床意义。方法选取手术切除并经病理证实的肾透明细胞癌及相应的癌旁标本45对,通过荧光定量PCR方法对45对ccRCC患者癌组织和癌旁正常组织的CDC25C mR-NA进行分析;运用免疫组化法对45对配对的癌组织和癌旁正常组织切片进行染色,检测CDC25C蛋白的表达;运用统计学方法分析CDC25C的表达量和患者的临床特点之间的关系。结果荧光定量PCR结果显示肾癌组织中CDC25C表达水平高于癌旁正常组织(P<0.01);对癌组织及癌旁正常组织切片免疫组化显示,癌组织CDC25C蛋白表达水平高于癌旁正常组织(P<0.01);同时发现CDC25C的表达水平与性别、年龄、肿瘤直径、TNM分期无关,与病理分级有关。结论 CDC25C可能参与了肾透明细胞癌的发生过程,CDC25C可能是肾透明细胞癌一个潜在的基因治疗靶点。更多还原

【Abstract】 Objective To investigate the expression level of CDC25C in clear cell renal cell carcinoma(ccRCC) and analyze its clinical significance.Methods 45 paired samples of ccRCCs and adjacent normal tissues were collected from patients who underwent radical nephrectomy.The expression of CDC25C in the 45 paired ccRCC and the adjacent normal tissues was tested in mRNA and protein level by q-PCR and immunohistochemistry respectively.Statistical analyses were applied to analyse the relationship between the level of CDC25C and clinical features.Results The mRNA level of CDC25C was significantly higher in ccRCC tissues compared with that in the adjacent normal tissues by q-PCR(P<0.01).Accordingly,the CDC25C protein expression was higher in ccRCC tissues than that in the adjacent normal tissues by immunohistochemistry(P<0.01).Moreover,there was no significant correlation between CDC25C expression and gender,age,tumor size and Tstage.Conclusion Our results indicate that CDC25C could play important roles in the development of ccRCC.CDC25C might be used as a potential genetic therapeutic target of ccRCC.更多还原