【摘要】 目的探讨新研制的DNA芯片应用于快速检测淋球菌16S rRNA基因突变的临床应用价值。方法根据淋球菌16SrRNA基因的序列信息设计探针并制作DNA芯片,PCR扩增并荧光标记包含16S rRNA基因突变的目的 DNA片段,与芯片杂交,同时以测序法为对照。结果 87份泌尿生殖道拭子均可被DNA芯片检测,同时检测发现有1株淋球菌16S rRNA基因突变(2709 C→T),与测序结果一致。结论DNA芯片检测淋球菌16SrRNA基因突变具有快速、高特异性和高灵敏度,可以应用于对大观霉素的耐药性临床检测。更多还原

【Abstract】 Objective To develop a new method,DNA chip,for rapid detection the mutations of neisseria gonorrhoeae 16S rRNA gene.Methods Probe were design according to the sequence of neisseria gonorrhoeae 16S rRNA genes,then DNA chip was made.DNA fragment which contains 16S rRNA gene mutation was amplified by PCR technique,labeled with cy5 fluorescence,and then hybridized with DNA chip.Results of DNA sequencing were used as the control.Results All of urogenital swab specimens were detected by DNA chip.We found one specimen which had mutation in gene 16S rRNA(2709 C→T),which was consistency between the DNA chip and DNA sequencing.Conclusion DNA chip is a rapid technique with high sensitivity and specificity for the detection of mutations of neisseria gonorrhoeae 16SrRNA gene.This method can be used for detection of spectinomycin resistance in clinical practice.更多还原