【摘要】 目的建立可控性强的病毒富集方法基本条件,科学评价空气微生物采样器的采样效率,为消毒监测和传染病监控提供依据。方法用气溶胶发生器模拟空气中流感病毒,用约30μm孔径超细静电纤维膜、0.2μm孔径Teflon静电膜和AGI-30大气采样器对病毒进行富集监测,用荧光定量PCR,分析不同采样器的不同采样条件对病毒的富集效能。结果超细静电纤维膜和Teflon膜对空气中流感病毒气溶胶的阻留吸附率98.0%以上,收集率介于74.6%～92.7%;AGI-30对空气中流感病毒的采样须≤20 Lpm,其阻留吸附率介于76.6%～88.9%,收集率介于59.3%～75.3%。结论采集空气中的流感毒,较大孔径和小孔径的静电膜都具有较高的富集效能,AGI-30的富集效能则较低,不宜用于长时间和较高流速采样。更多还原

【Abstract】 Objective To establish stronger controllable basic conditions for virus enrichment methods,and scientifically evaluate the sampling efficiency of microbial air sampler,so as to provide evidence for sterilization surveillance and infectious disease monitoring.Method Through the aerosol generator,influenza virus was simulatied into the air.The concentrated virus was monitored by aperture approximately with 30μm superfine static electricity fiber membrane,aperture 0.2μm Teflon static electricity membrane and the AGI-30 atmosphere sampling instrument.Then fluorescence quota PCR was usd to analyze and discuss the efficiency.Result In different sampling conditions(membrane surface <450Pa),the influenza virus aerosol filtration and collection rate of ultra-fine fiber electrostatic membrane and Teflon membrane is over 98%,The range of collection rate is from 74.6% to 92.7%.When using AGI-30,the sampling flow rate must be under 20 Lpm,while the range of filtration and collection rate is from 76.6% to 88.9% and the collection rate 59.3%-75.3%.Conclusion For acquisition of influenza virus in the air,both the large aperture and small aperture electrostatic membrane have higher enrichment efficiency.On the other hand,the AGI-30 enrichment efficiency is low,and should not be used for a long time with a high flow rate sampling.更多还原