【摘要】 目的探讨西红花酸对Aβ₂5-35诱导的SD大鼠海马神经元损伤的神经保护作用。方法常规培养SD大鼠海马神经元,分为对照组、0.1μM西红花酸组、10μmol/L Aβ₂5-35组、0.01μM西红花酸+10μmol/L Aβ₂5-35组、0.1μM西红花酸+10μmol/L Aβ₂5-35组、1μM西红花酸+10μmol/L Aβ₂5-35组;MTT检测细胞活力变化情况;荧光探针DCFH-DA检测细胞内ROS水平变化情况。结果 10μmol/L Aβ₂5-35显著降低细胞的活性和升高海马神经元ROS水平（P<0.01）;不同浓度西红花酸（0.01、0.1和1μM）预处理后,细胞活性分别比Aβ₂5-35组提高了39.2%、56.1%和76.4%,差异有显著性意义（P<0.05）,培养10天和25天海马神经元的ROS水平与Aβ₂5-35组比较均显著降低（P<0.01）。结论西红花酸对Aβ₂5-35诱导海马神经元的损伤具有保护作用,能显著降低Aβ₂5-35引起的海马神经元ROS水平的升高。更多还原

【Abstract】 Objective To explore the neuroprotective effects of crocetin on amyloid β25-35 -induced neurotoxicity in cultured hippocampal neurons. Methods Hippocampal neurons derived from 4 weeks old SD rats were cultured and divided into six groups: the control group, 0.1 μM crocetin group, 10 μmol/L Aβ25-35 group, 0.01 μM crocetin +10 μmol/L Aβ25-35 group, 0.1 μM crocetin +10 μmol/L Aβ25-35 group, 1 μM crocetin +10 μmol/L Aβ25-35 group. Cell viability and ROS levels in hippocampal neurons were respectively detected by MTT assay and fluorescent probe DCFH-DA. Results After treatment with 10 μmol/L Aβ25-35 , cell viability significantly decreased and ROS levels significantly increased (P <0.01). However, cell viability of the group pre-cultured with crocetin significantly increased (P <0.05 compared with Aβ25-35 group), and ROS levels significantly decreased (P <0.01 compared with Aβ25-35 group). Conclusion Crocetin can attenuate Aβ25-35 -induced neuronal damage, increase cell viability and decrease ROS levels in cultured hippocampal neurons of SD rats.更多还原