【摘要】 目的 建立测定人血浆中白消安浓度的柱前衍生高效液相色谱法。方法 以1,5-戊二醇二甲磺酸酯为内标,血浆样品经二乙基二硫代氨基甲酸钠衍生化处理后,以乙腈-0.7%冰醋酸水溶液（69:31）为流动相,流速1.0 ml·min^-1,采用Xterra^?RP18色谱柱分离,在280nm波长下进行检测。结果 白消安线性范围为0.20～4.00 mg·L^-1,以加权最小二乘法计算得到回归方程为y=2.56x+16.7（r=0.999 3,n=7）,提取回收率81.01%～82.72%,方法回收率96.50%～97.90%,日内、日间精密度（RSD）均小于10%。最低血浆检测质量浓度为0.080 mg·L^-1。结论 该方法准确、灵敏,适用于白消安血浆浓度测定及其药代动力学研究。更多还原

【Abstract】 Objective To establish a method for determination of plasma busulfan concentration by pre-column derivatization pre-high performance liquid chromatography.Methods 1,5-diol dimethyl sulfonate was used as internal standard,plasma samples were processed by derivatization with acetonitrile-0.7% acetic acid（69:31） as mobile phase,at a flow rate of 1 ml·min^-1,Xterra^? RP18 column（5 μm,4.6 mm ×250 mm） was used,at 280 nm wavelength detection.Results Busulfan plasma concentration of the linear range of 0.20-4.00 mg·L^-1,computing the weighted least squares regression equation for the y=2.56x+16.7（r=0.999 3,n=7）.The extraction recovery was81.01%-82.72% and the method recovery was 96.50%-97.90%.The intra-and inter-day RSD were less than10%.Minimum plasma concentration of detection was 0.080 mg·L^-1.Conclusion This method is accurate,sensitive and suitable for determination of busulfan plasma concentration and pharmacokinetic studies.更多还原