【摘要】 目的 观察多次腹腔注射丙泊酚（propofol,P）对小鼠自主活动和学习记忆能力的影响。方法 昆明种小鼠90只,分为镇静和记忆两实验大组,每实验大组再分为3组（n=15）:NS组腹腔注射生理盐水,另两组分别腹腔注射丙泊酚10 mg/kg(P₁₀组)、15 mg/kg（P₁5组）。用自主活动仪检测每组小鼠第1、2、3天第2次给药15 min后5 min内的自主活动次数,用跳台仪观察小鼠最后1次给药后24、48、72 h的跳台潜伏期（step-down latency）和错误次数（error times）。结果 镇静实验结果显示,在第1、2、3天,P₁₀组小鼠自主活动次数与NS组比较差异无统计学意义（P>0.05）,而P₁₅组活动减少（P<0.01或P<0.05）;每组第1、2、3天之间差异没有统计学意义（P>0.05）。记忆实验结果显示:在24 h以及48 h,P₁₀组、P₁₅组与NS组相比跳台潜伏期缩短（/P<0.01或P<0.05）、错误次数增多（P<0.05）;72 h时,3组潜伏期和错误次数的差异均无统计学意义（P>0.05）。结论小鼠对丙泊酚产生的遗忘作用敏感性高于其产生的镇静作用;多次应用丙泊酚会产生遗忘作用,但不会持续到48 h。更多还原

【Abstract】 Objective To observe the impacts of propofol（P） by intraperitoneal injection（ip） repeatedly on autonomic activities and learning and memory in mice.Methods 90 Kunming mice were randomly divided into 2 great test groups:sedative test group and memory test group.Each great test group were divided futher into 3 small groups:NS group（ip NS）,P₁₀ group(ip propofol 10 mg·kg^-1),P₁₅ group(ip propofol 15 mg·kg^-1).The autonomic activities apparatus was used to observe the autonomic activities in 5 min of each group after 15 min by ip medicine at the 1st,2nd and 3rd day;the step-down apparatus was used to observe the step-down latency and error times at 24 h,48 h,72 h after the last ip medicine.Results In sedative test,at the 1st,2nd and 3rd day,compared with NS group,the autonomic activities of P₁₀ group had no statistically significant difference（P>0.05）,but that of P₁₅ group decreased（P <0.01 or P <0.05）;there were no statistical significances at the 1 st,2nd or 3rd day with each group（P> 0.05）.In the memory test,compared with NS group,at 24 h or 48 h,step-down latency shortened（P <0.01 or P <0.05） and the error times decreased（P <0.05） of P₁₀ group or P₁₅ group,but that of the three groups had no statistically significant difference at 72 h（P> 0.05）.Conclusion Mice are more sensitive to the forgotten effects than the sedation of propofol;repeated applications of propofol can produce forgotten effects but can’t last 48 hours.更多还原