【摘要】 目的采用实时定量PCR方法检测食管癌样本中Smad4基因mRNA的表达量,分析其与临床资料之间的关系。方法设计Smad4基因特异性探针和引物,建立Smad4基因实时定量检测方法;检测40例食管癌患者(男28例,女12例,38~82岁,中位年龄60岁)肿瘤切除石蜡标本中Smad4基因mRNA的模板拷贝数,以beta-actin为内参得出校正过的基因表达相对值。统计学分析这些值与临床病理及预后的关系。结果 Smad4的mRNA表达水平与性别、年龄、淋巴结转移、病理亚型无关(P>0.05);与细胞分化和病理分期有关(P<0.05)。Smad4低表达的患者预后较高表达者差(P=0.009)。结论食管癌中Smad4mRNA表达的可作为预测淋巴结转移、评价预后的一个重要指标。Smad4可能是食管癌变过程中的重要分子。更多还原

【Abstract】 Objective To detect the expression level of smad4 mRNA in the sample of esophageal carcinoma by using real-time quantitative PCR method analyze its relationship with clinical materials.Methods To design specific probe and primer for gene Smad4 and to establish real-time quantitative PCR method for Smad4 as well.Tumor paraffin specimens of 40 esophageal carcinoma patients(28 males and 12 females,age from 38 to 82 years old,median 60 years old) were analyzed in index of template copy number,and the relative value of gene expression was counted by beta-actin regulation.Relationship between these values and clinicopathology and prognosis were analyzed statistically.Results The expression level of smad4 mRNA was positively correlated to pathologic stage and cell differentiation(P<0.05),but not correlated to age,gender,lymphatic metastasis and pathological subtypes(P>0.05).Conclusion Smad4 mRNA in esophageal carcinoma may be an important indicator in predicting lymphatic metastasis and evaluating the prognosis.更多还原