【摘要】 目的建立一种人神经生长因子(NGF)mRNA荧光定量聚合酶链反应(PCR)检测方法,探讨接触时间对正己烷接触人群NGF mRNA表达水平的影响。方法以接触正己烷工龄分组。设计引物、探针,检测对照组和接触正己烷各组之间的NGF mRNA水平。结果利用该荧光定量PCR检测结果显示工龄<2年组、2～6年组、>6年组以及对照组的NGF mRNA以10为底的对数分别为(8.88±0.08)、(8.68±0.08)、(8.53±0.11)和(8.90±0.07)copies/L。2～6年组和>6年组的NGF mRNA表达水平均低于对照组。各组内男女之间NGF mRNA表达水平比较,差异无统计学意义(P>0.05)。结论正己烷的毒性对于NGF mRNA的表达有明显的时间依赖关系,随着接触时间加长,NGF的表达逐渐减弱;所建立的检测方法特异性强,完全符合检测要求。更多还原

【Abstract】 Objective To study the influence on n-hexane exposure time for the nerve growth factor(NGF) mRNA expression level of workers exposed to n-hexane by establishing a detection method of real-time PCR technology. Methods Workers exposed to n-hexane were grouped according to exposure time.The NGF mRNA expression level of workers exposed to n-hexane and the control group were respectively detected by the real-time PCR technology,by primer and probe devised. Results The NGF mRNA expression level of less than 2 years service group,2-6 years of service group,more than 6 years seniority group and the control group by the base 10 logarithm of were respectively(8.88±0.08),(8.68±0.08),(8.53±0.11)and(8.90±0.07)copies/L.The NGF mRNA expression level of 2-6 years of service group and more than 6 years seniority group was higher than which of the control group.The NGF mRNA expression level wasn’t statistically significant difference between men and women within each groups(P>0.05). Conclusion There were the negative correlation of time-dependent between the NGF mRNA expression level of workers exposed to n-hexane and n-hexane toxicity.The NGF mRNA expression level was gradually weakened with the increasing of exposure time.The specificity of the real-time PCR technology developed which was fully consistent with testing requirements was highly.更多还原