【摘要】 目的:探讨七氟醚预处理对大鼠心脏缺血-再灌注损伤(IRI)中细胞凋亡的影响及Akt/NF-κB表达水平的影响。方法:选择健康SD大鼠30只,按随机数字表法分为假手术组(P组)、IR组和七氟醚预处理组(S组),每组各10只。P组不进行任何IR处理。S组予吸入2%七氟醚30 min,洗脱15 min,IR组在该45 min内不作预处理的,然后阻断两组大鼠冠状动脉前降支20 min,随后再灌注2 h。采用TUNEL法检测原位凋亡细胞,计算细胞凋亡指数。采用蛋白免疫印迹法检测p-Akt和NF-κB蛋白表达水平。结果:与P组相比,IR组和S组的细胞凋亡指数增加,p-Akt和NF-κB蛋白表达明显上调(P均<0.01);与IR组比较,S组的细胞凋亡指数下降,p-Akt蛋白表达水平进一步升高,NF-κB蛋白表达水平则下降(P均<0.01)。结论:七氟醚预处理能够抑制IR所致的心肌细胞凋亡,其作用机制可能与上调p-Akt表达,抑制NF-κB的活性有关。更多还原

【Abstract】 Objective:To investigate the effects of Sevoflurane pretreatment on myocardial apoptosis and Akt/NF-κB expressions during ischemia/reperfusion(I/R) injury in rats.Methods:Thirty rats were randomly divided into three groups with 10 rats in each group:sham operation group;I/R group;Sevoflurane group.Rats in sham operation group underwent no I/R.In I/R group,rats were sujected to 20 minutes of ischemia followed by 2 hours of reperfusion.In Sevoflurane group,rats inhaled 2% sevoflurane for 30 minutes followed by 15 minutes of wash-out before I/R.Myocardial apoptosis was detected by TUNEL method.Western blot was used to examine the expression of p-Akt and NF-κB.Results: Compared with sham operation group,apoptosis index and expression of p-Akt and NF-κB increased in I/R group and Sevoflurane group(P<0.01).Compared with I/R group,apoptosis index and expression of NF-κB decreased in Sevoflurane group(P<0.01),but expression of p-Akt increased(P<0.01).Conclusion:Sevoflurane pretreatment could inhibit apoptosis in ischemia-reperfusion heart in rat,which may be related to up-regulation of p-Akt and down-regulation of NF-κB.更多还原